Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
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چکیده
منابع مشابه
Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purifi...
متن کاملMolecular cloning, expression and characterization of a functional GSTmu class from the cattle tick Boophilus annulatus.
A full-length cDNA of a glutathione S-transferase (GST) was cloned from a cDNA library of the local Egyptian cattle tick Boophilus annulatus. The 672 bp cloned fragment was sequenced and showed an open reading frame encoding a protein of 223 amino acids. Comparison of the deduced amino acid sequence with GSTs from other species revealed that the sequence is closely related to the mammalian mu-c...
متن کاملIdentification of Tropomyosin and Its Immunological Properties from Larvae of Cattle Tick, Boophilus annulatus
BACKGROUND Boophilus annulatus is an obligate blood feeder tick that can cause great losses in animals due to anemia and its ability to injure its host skin directly. The aim of this study was identification of cattle humoral immune response to some tick proteins during experimental infestation. METHODS Immune sera against tick were collected from experimentally infested cattle with ticks. On...
متن کاملidentification of tropomyosin and its immunological properties from larvae of cattle tick, boophilus annulatus.
background : boophilus annulatus is an obligate blood feeder tick that can cause great losses in animals due to anemia and its ability to injure its host skin directly. the aim of this study was identification of cattle humoral immune response to some tick proteins during experimental infestation. methods : immune sera against tick were collected from experimentally infested cattle with ticks. ...
متن کاملAnalysis of Immunogenic Relevant Proteins in Rhipicephalus (Boophilus) annulatus Tick
BACKGROUND Considering the importance of ticks as a main group transmitting pathogen organisms, this study designed to recognize immunogenic proteins in different tissues of Rhipicephalus (Boophilus) annulatus tick and to find out if there are common proteins in these tissues. METHODS Seven cattle were experimentally infested with about 10000 R. annulatus larvae and their humoral immune respo...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2007
ISSN: 1229-845X
DOI: 10.4142/jvs.2007.8.2.175